Journal: Veterinary microbiology

Article Title: Induction of innate host responses characterized by production of interleukin (IL)-1β and recruitment of macrophages to the respiratory tract of chickens following infection with infectious bronchitis virus (IBV).

doi: 10.1016/j.vetmic.2018.01.001

Figure Lengend Snippet: Fig. 1. Photomicrographs (40x magnification) of H&E stained tracheal sections from IBV infected and uninfected control chickens at 1, 2, 3, 5 and 7 dpi are shown. Loss of cilia, epithelial metaplasia, and numerous in- flammatory cell infiltration can be seen in the infected tracheas compared to uninfected control tracheas.

Article Snippet: The primary antibody specific to chicken IL-1β (anti-IL-1β) raised in rabbits was used in a 1:10 dilution (ABD Serotec, Kidlington, UK).

Techniques: Staining, Infection, Control

Journal: Veterinary microbiology

Article Title: Induction of innate host responses characterized by production of interleukin (IL)-1β and recruitment of macrophages to the respiratory tract of chickens following infection with infectious bronchitis virus (IBV).

doi: 10.1016/j.vetmic.2018.01.001

Figure Lengend Snippet: Fig. 2. Photomicrographs (40x magnification) of H&E stained lung sec- tions from IBV infected and uninfected control chickens at 1, 2, 3, 5 and 7 dpi are shown. Inflammatory cell infiltration and haemorrhage into the parabronchi and parabronchial lumen can be seen in the infected lungs compared to uninfected control Lungs.

Article Snippet: The primary antibody specific to chicken IL-1β (anti-IL-1β) raised in rabbits was used in a 1:10 dilution (ABD Serotec, Kidlington, UK).

Techniques: Staining, Infection, Control

Journal: Veterinary microbiology

Article Title: Induction of innate host responses characterized by production of interleukin (IL)-1β and recruitment of macrophages to the respiratory tract of chickens following infection with infectious bronchitis virus (IBV).

doi: 10.1016/j.vetmic.2018.01.001

Figure Lengend Snippet: Fig. 3. The IBV genome loads ( ± SEM) per 200 ng RNA extracted from trachea and lungs at 1, 2, 3, 5 and 7 dpi as determined using a real-time PCR assay targeting the IBV N gene are shown. Fig. 3a and b correspond to the genome loads in the trachea and lung, re- spectively (Statistical significance: *p < 0.05, **p < 0.01).

Article Snippet: The primary antibody specific to chicken IL-1β (anti-IL-1β) raised in rabbits was used in a 1:10 dilution (ABD Serotec, Kidlington, UK).

Techniques: Real-time Polymerase Chain Reaction

Journal: Veterinary microbiology

Article Title: Induction of innate host responses characterized by production of interleukin (IL)-1β and recruitment of macrophages to the respiratory tract of chickens following infection with infectious bronchitis virus (IBV).

doi: 10.1016/j.vetmic.2018.01.001

Figure Lengend Snippet: Fig. 4. Quantitative data of the average areas (as a portion of the total size of the section) stained with red (macrophages) (Fig. 4a), green (IL-1β) (Fig. 4b) and yellow fluorescence (IL-1β positive macrophages) (Fig. 4c) along with the representative micrographs (60x magnification) of infected and uninfected tracheal sections originated at 1, 2, 3, 5 and 7 dpi are shown (Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The primary antibody specific to chicken IL-1β (anti-IL-1β) raised in rabbits was used in a 1:10 dilution (ABD Serotec, Kidlington, UK).

Techniques: Staining, Infection

Journal: Veterinary microbiology

Article Title: Induction of innate host responses characterized by production of interleukin (IL)-1β and recruitment of macrophages to the respiratory tract of chickens following infection with infectious bronchitis virus (IBV).

doi: 10.1016/j.vetmic.2018.01.001

Figure Lengend Snippet: Fig. 5. Quantitative data of the average areas (as a portion of the total size of the section) stained with red (macrophages) (Fig. 5a), green (IL-1β) (Fig. 5b) and yellow fluorescence (IL-1β positive macrophages) (Fig. 5c) along with the representative micrographs (60x magnification) from infected and uninfected lung sections originated at 1, 2, 3, 5 and 7 dpi are shown (Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The primary antibody specific to chicken IL-1β (anti-IL-1β) raised in rabbits was used in a 1:10 dilution (ABD Serotec, Kidlington, UK).

Techniques: Staining, Infection

Journal: Applied microbiology and biotechnology

Article Title: A new standardization for the use of chicken embryo: selection of target from the phage display library and infection.

doi: 10.1007/s00253-024-13227-x

Figure Lengend Snippet: Fig. 1 Percentage of embryos killed with different doses of SP at two different ages. NC, negative control group with PBS; 6 log/embryo, group inoculated with 6 log/embryo of SP; 4 log/embryo, group inoc- ulated with 4 log/embryo of SP; 2 log/embryo, group inoculated with 2 log/embryo of SP

Article Snippet: Then, we incubate the plates with the antibodies, rabbit anti-chicken IFN-γ IgG antibody (BioRad), rabbit anti-chicken IL-1β IgG antibody (BioRad) or IL-10 Polyclonal IgG antibody (Thermo), diluted (1:500) in 3% BSA + PBS for 1 h at 37 °C.

Techniques: Negative Control